CD7 Antibody

• Catalog Number: orb1523070
• Category: Antibodies
• Description: CD7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 190 and 240
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CD7
• UniProt ID: P09564
• NCBI: NP_006128.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: T-cell surface antigen Leu-9; Tp40; T-cell leukemi
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CD7 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN or RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD7 antibody orb1523070 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human CD7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD7 antibody orb1523070 used for IP at 6 µg per reaction.

Detection of human CD7 by immunohistochemistry.Sample: FFPE section of human stomach cancer infiltrating lymphocytes. Antibody: Affinity purified rabbit anti-CD7 (Cat. No. orb1523070) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB