CD68 (mouse specific) Rat Monoclonal Antibody

SKU: orb1294245

Description

Rat Monoclonal antibody to mouse CD68

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IHC
• Dilution Range: Immunohistochemistry (Frozen) 1:50-1:100, Flow Cytometry 1:50-1:100
• Reactivity: Mouse
• Application Notes:
  Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user

Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Clone No.: [FA-11]
• Target: CD68 (mouse specific)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate-buffered solution, pH 7.2, containing 0.025% ProClin 300 , 20% Glycerol
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Mouse Frozen-embedded tonsil tissue. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes and permeabilized with 0.5% Triton X-100. Then, the tissue was stained with orb1294245 mouse CD68 [FA-11] monoclonal antibody at 1-100, 4°C overnight.

Mouse peripheral blood monocytes intracellularly stained with orb1294245 mouse CD68 [FA-11] monoclonal antibody.

Quick Database Links

Gene Symbol

CD68 (mouse specific)