CD64 antibody

• Catalog Number: orb154423
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD64 which is a glycoprotein that is constitutively expressed on human monocytes and macrophages. CD64 plays a putative role in the initiation of cell-mediated cytotoxicity and also acts in mediating humoral immune responses.
• Clonality: Monoclonal
• Clone Number: 10.1
• Tested applications: FA, FC, ICC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human, Primate
• Isotype: Mouse IgG1 kappa
• Immunogen: Rheumatoid synovial fluid cells and fibronectin purified human monocytes
• Concentration: 1 mg/ml
• Dilution range: Functional application: Blocking of IgG binding to the FcγR1. Flow cytometry: Recommended dilution: 1-4 μg/ml. Immunohistochemistry (paraffin sections and frozen sections): There can occur problems with paraformaldehyde fixation.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD64
• Entrez: 2209
• UniProt ID: P12314
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: Anti-FcRI antibody, anti-IGFR1 antibody, anti-FcgR
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Separation of human monocytes (red-filled) from CD64 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD64 (10.1) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD64 (10.1) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.