CD64 antibody (Biotin)

• Catalog Number: orb222723
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD64 conjugated to Biotin
• Clonality: Monoclonal
• Clone Number: 10.1
• Tested applications: FC, ICC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human, Primate
• Isotype: Mouse IgG1 kappa
• Immunogen: Rheumatoid synovial fluid cells and fibronectin purified human monocytes
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 4 μg/ml.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: CD64
• Entrez: 2209
• UniProt ID: P12314
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: FcgammaRI
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD64 (10.1) biotin antibody (concentration in sample 4 μg/ml, Streptavidin APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD64 (10.1) biotin antibody (concentration in sample 4 μg/ml, Streptavidin APC).