CD63 antibody (Biotin)

• Catalog Number: orb154462
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to Biotin which recognizes is a CD63 that is lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is observed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. CD63 is belongs to the TM4 superfamily of leukocyte glycoproteins. CD63 may participates in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome.
• Clonality: Monoclonal
• Clone Number: MEM-259
• Tested applications: FC, ICC, IHC-P, IP
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: HPB-ALL T cell line
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-3 μg/ml; positive material: activated platelets, neutrophils and basophils.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: CD63
• Entrez: 967
• UniProt ID: P08962
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-OMA81H antibody, anti-Granulophysin antibody,
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-3 μg/ml; positive material: activated platelets, neutrophils and basophils.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of IgE stimulated peripheral blood mononuclear cells stained using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0.6 μg/ml, Streptavidin APC) and anti-human CD203c (NP4D6) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of CD63 positive CD203c positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of IgE stimulated human peripheral whole blood using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0.6 μg/ml, Streptavidin APC).

Flow cytometry surface staining pattern of IgE stimulated human peripheral whole blood stained using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0.6 μg/ml, Streptavidin APC).