CD62P/SELP Antibody

• Catalog Number: orb251546
• Category: Antibodies
• Description: CD62P/SELP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CD62P recombinant protein (Position: W42-G271). Human CD62P shares 75.2% and 75.7% amino acid (aa) sequence identity with mouse and rat CD62P, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: ELISA , 0.1-0.5μg/ml, HumanImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, HumanFlow Cytometry, 1-3μg/1x106 cells, HumanImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 90834 MW
• UniProt ID: P16109
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: P-selectin;CD62 antigen-like family member P;Granu
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of THP-1 cells using anti-P-Selectin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CD62P using anti-CD62P antibody.Lane 1:A549 Cell;2:K562 Cell.

IHC analysis of CD62P using anti-CD62P antibody. CD62P was detected in paraffin-embedded section of Human Tonsil Tissue.