CD62L/SELL Antibody

• Catalog Number: orb251572
• Category: Antibodies
• Description: CD62L/SELL Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human L-selectin recombinant protein (Position: W39-Y372). Human L-selectin shares 77.5 % and 78.7 % amino acid (aa) sequence identity with mouse and rat L-selectin, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat ELISA , 0.1-0.5μg/ml, Human, -Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 42187 MW
• UniProt ID: P14151
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: L-selectin;CD62 antigen-like family member L;Leuko
• Note: For research use only
• Application notes: WB: The detection limit for L-selectin is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

IHC(P) analysis of Human Tonsil Tissue using Anti-L-selectin Picoband antibody.

Western blot analysis using Anti-L-selectin Picoband antibody.Lane 1:Human Placenta Tissue;2:JURKAT Cell;3:CEM Cell;4:HL60 Cell;5:Rat Spleen Tissue;6:Mouse Spleen Tissue.