CD62L antibody

• Catalog Number: orb222764
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD62L
• Clonality: Monoclonal
• Clone Number: DREG56
• Tested applications: FA, FC, IHC-Fr, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: PMA-activated human peripheral blood leukocytes
• Concentration: 1 mg/ml
• Dilution range: Functional application: Particularly effective in blocking of lymphocyte CD62L-mediated binding to peripheral lymph node HEV. Flow cytometry: Recommended dilution: 2 μg/ml. Western blotting: Non-reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD62L
• Entrez: 6402
• UniProt ID: P14151
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: L-Selectin
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62L (DREG56) purified antibody (low endotoxin, concentration in sample 1 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (low endotoxin, concentration in sample 1 μg/ml) GAM APC.