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Catalog Number | orb248235 |
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Category | Antibodies |
Description | CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular “scavenger receptor cysteine-rich” (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. antibody to CD6 can be used to deplete T cells from bone marrow transplants to prevent graft versus host disease. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | C6/372 + 3F7B5 |
Tested applications | IF, IHC-P |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | Human recombinant CD6 protein (C6/372) and the rheumatoid synovial T cell line ST-1 (3F7B5) were used as the immunogen for this antibody cocktail. |
Dilution range | Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This CD6 antibody is available for research use only. |
Entrez | 923 |
Storage | Store the CD6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human tonsil (10X) tissue using CD6 Cocktail antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human tonsil (20X) tissue using CD6 Cocktail antibody
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