CD58 monoclonal antibody (M02), clone 3B12-D5

• Catalog Number: orb2294747
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full-length recombinant CD58.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3B12-D5
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG2a Kappa
• Immunogen: CD58 (AAH05930, 1 a.a. ~ 240 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MVAGSDAGRALGVLSVVCLLHCFGFISCFSQQIYGVVYGNVTFHVPSNVPLKEVLWKKQKDKVAELENSEFRAFSSFKNRVYLDTVSGSLTIYNLTSSDEDEYEMESPNITDTMKFFLYVLESLPSPTLTCALTNGSIEVQCMIPEYYNSHRGLIMYSWDCPMEQCKRNSTSIYFKMENDLPQKIQCTLSNPLFNTTSSIILTTCIPSSGHSRHRYALIPIPLAVITTCIVLYMNGMYAF
• NCBI: AAH05930
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged CD58 is 1 ng/ml as a capture antibody.

Western Blot analysis of CD58 expression in transfected 293T cell line by CD58 monoclonal antibody (M02), clone 3B12-D5. Lane 1: CD58 transfected lysate (Predicted MW: 28.1 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (52.14 KDa).