B3GAT1 Antibody

SKU: orb1252548

Description

B3GAT1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  Western blot: 2-4 µg/mLFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 2-4 µg/mL for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the CD57 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgM, kappa
• Clone No.: HNK-1 or Leu-7
• Immunogen: Human peripheral blood mononuclear cells were used as the immunogen for the CD57 antibody.
• Target: B3GAT1
• Purification: PEG precipitation
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Beta-1,3-glucuronyltransferase 1, Glucuronosyltransferase P, GlcAT-P, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase, GlcUAT-P, B3GAT1, GLCATP

Formalin-fixed, paraffin-embedded human tonsil stained with CD57 antibody (HNK-1).

Western blot testing of K562 cell lysate with CD57 antibody (clone HNK-1). Predicted molecular weight: ~38/110kDa (unmodified/glycosylated).

Quick Database Links

Gene Symbol

B3GAT1

UniProt

Q9P2W7