CD57 Antibody [HNK-1 or Leu-7]

• Catalog Number: orb1252548
• Category: Antibodies
• Description: CD57 Antibody [HNK-1 or Leu-7]
• Target: B3GAT1
• Clonality: Monoclonal
• Isotype: IgM, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: PEG precipitation
• Immunogen: Human peripheral blood mononuclear cells were used as the immunogen for the CD57 antibody.
• UniProt ID: Q9P2W7
• Tested applications: FC, IF, IHC-P, WB
• Application notes: Western blot: 2-4 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the CD57 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: HNK-1 or Leu-7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Galactosylgalactosylxylosylprotein 3-beta-glucuron
• Research Area: Immunology & Inflammation
• Note: For research use only

Formalin-fixed, paraffin-embedded human tonsil stained with CD57 antibody (HNK-1).

Western blot testing of K562 cell lysate with CD57 antibody (clone HNK-1). Predicted molecular weight: ~38/110kDa (unmodified/glycosylated).