CD55 Antibody

• Catalog Number: orb1671977
• Category: Antibodies
• Description: CD55 Antibody
• Clonality: Recombinant
• Clone Number: LU30
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG lambda
• Immunogen: Human CD55.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CD55
• UniProt ID: P08174
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: DAF, Complement decay-accelerating factor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human lung tissue using anti-CD55 antibody LU30 Anti-CD55 (Complement decay-accelerating factor) staining of paraffin embedded human lung tissue using the rmeric version of LU30 (orb1671977). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer. Primary antibody incubation with orb1671977 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody for 20 mins followed by Ddiaminobenzidine- and counter staining with haematoxylin. Strong staining of the epithelial surface of alveoli may be observed. Recommended concentration- 2-4 ug/ml.

Flow-cytometry using the anti-CD55 (Complement decay-accelerating factor) antibody LU-30. U93 cells were stained with unimmunized r antibody (black line) or the rmeric version of LU-30 (orb1671977 - blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.