CD54 antibody (PE)

• Catalog Number: orb43582
• Category: Antibodies
• Description: Mouse Monoclonal to CD54.
• Clonality: Monoclonal
• Clone Number: 1H4
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Raji cells and spleen cells fused with NS1 cells
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD54
• Entrez: 3383
• UniProt ID: P05362
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD54 antibody, anti-ICAM-1 antibody
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD54 (1H4) PE antibody (20 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD54 (1H4) PE antibody (20 µl reagent / 100 µl of peripheral whole blood).