CD50 antibody

• Catalog Number: orb154422
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD50 which is also known as ICAM-3, is belongs to the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 and alphad/beta2. ICAM-3 is expressed on leukocytes and epidermal Langerhans cells. It can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes.
• Clonality: Monoclonal
• Clone Number: MEM-04
• Tested applications: FA, FC, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b kappa
• Immunogen: Leukocytes of a patient suffering from LGL-type leukemia.
• Concentration: 1 mg/ml
• Dilution range: Functional application: The antibody MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1). Flow cytometry: Recommended dilution: 1-4 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: tonsil.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD50
• Entrez: 3385
• UniProt ID: P32942
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: Anti-ICAM-3 antibody, anti-ICAM-R antibody
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Separation of leukocytes stained using anti-human CD50 (MEM-04) purified antibody (concentration in sample 3 µg/ml, GAM APC, red-filled) from leukocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD50 (MEM-04) purified antibody (concentration in sample 3 µg/ml, GAM APC).

Immunohistochemistry staining of human tonsil (paraffin sections) using anti-CD50 (MEM-04).