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Catalog Number | orb2294932 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant CD5. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 2F7 |
Tested applications | ELISA, WB |
Reactivity | Human |
Isotype | IgG2a Kappa |
Immunogen | CD5 (ABM83003, 403 a.a. ~ 495 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | KKLVKKFRQKKQRQWIGPTGMNQNMSFHRNHTATVRSHAENPTASHVDNEYSQPPRNSRLSAYPALEGVLHRSSMQPDNSSDSDYDLHGAQRL |
NCBI | ABM83003 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
CD5 monoclonal antibody (M01), clone 2F7. Western Blot analysis of CD5 expression in K-562.
Detection limit for recombinant GST tagged CD5 is 0.3 ng/ml as a capture antibody.
Western Blot analysis of CD5 expression in transfected 293T cell line by CD5 monoclonal antibody (M01), clone 2F7. Lane 1: CD5 transfected lysate (Predicted MW: 36.3 KDa). Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (35.86 KDa).