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Catalog Number | orb154491 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody conjugated to FITC which recognizes CD5 that also designated Lyt-1 that has been identified as a transmembrane glycoprotein that is expressed on 70% of normal peripheral blood lymphocytes and on virtually all T lymphocytes in thymus and peripheral blood. It plays role as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2. CD5 is expressed at various developmental and activation stages on human B cells. |
Clonality | Monoclonal |
Clone Number | L17F12 |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgG2a kappa |
Immunogen | Human acute lymphoblastic leukemia (ALL) T cells |
Dilution range | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | FITC |
Target | CD5 |
Entrez | 921 |
UniProt ID | P06127 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-T1 antibody, anti-LEU1 antibody Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Separation of human CD5 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD5 (L17F12) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD5 (L17F12) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).
ELISA, ICC, IHC-Fr, IP, Multiplex Assay | |
Mouse | |
Rat | |
Monoclonal | |
FITC |
ELISA, FC, ICC, IHC-Fr, IP, Multiplex Assay | |
Mouse | |
Rat | |
Monoclonal | |
FITC |
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