CD49f Antibody

• Catalog Number: orb1147812
• Category: Antibodies
• Description: CD49f (alpha 6 integrin) is a type I transmembrane glycoprotein, which noncovalently associates with CD29 (beta 1 integrin) to form VLA-6, and with CD104 (beta 4 integrin) to form alpha6/beta4 integrin complex. CD49f is expressed on platelets, megakaryocytes, monocytes, T cells and thymocytes. It is widely expressed on many cultured adherent cell lines and on epithelia in non-lymphoid tissues. It is important for adhesion to laminins, invasin, and merosin, hence also for cell migration, embryogenesis, metastasis, formation of hemidesmosomes in epithelia, and other processes.
• Clonality: Monoclonal
• Clone Number: GoH3
• Tested applications: FC, ICC, IHC-Fr, IP
• Reactivity: Bovine, Canine, Equine, Feline, Human, Mouse, Porcine, Primate, Rabbit, Sheep
• Isotype: Rat IgG2a
• Immunogen: mouse mammary tumor cells
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Purity: Purified by protein-G affinity chromatography.
• Conjugation: Unconjugated
• Target: CD49f
• Entrez: 3655
• UniProt ID: P23229
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: ITGA6, VLA6alpha, platelet gpIc, integrin subunit
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of human blood thrombocytes stained using anti-CD49f (GoH3) purified antibody (concentration in sample 1.7 μg/ml, GAM APC, red-filled) from blood thromobocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD49f (GoH3) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).