CD46 Antibody (monoclonal, 9E9)

• Catalog Number: orb623770
• Category: Antibodies
• Description: CD46 Antibody (monoclonal, 9E9)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 9E9
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CD46 (366-392aa YRYLQRRKKKGTYLTDETHREVKFTSL).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 50-70 kDa
• UniProt ID: P15529
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: AHUS2 antibody; Antigen defined by monoclonal anti
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of PBMC cells using anti-CD46 antibody(Blue line).Isotype control antibody (Green line) was mouse IgG.Unlabelled sample (Red line) was also used as a control.

IHC analysis of CD46 using anti-CD46 antibody.

IHC analysis of CD46 using anti-CD46 antibody.

WB analysis using anti ZO-1 antibody.Lane 1:HepG2 tissue