CD45RO Antibody (T-cell marker)

• Catalog Number: orb385702
• Category: Antibodies
• Description: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Human T lymphocytes were used as immunogen for this CD45RO antibody.
• UniProt ID: P08575
• Tested applications: FACS, IHC-P
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Application notes: Due to variation in protocol and secondary antibody used, the CD45RO antibody may need to be titered for optimal performance.1. FFPE staining requires 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: T200/797
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics, Infectious Diseases
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC testing of CD45RO antibody and FFPE human tonsil. HIER: boil tissue sections in 10mM citrate buffer, pH6 for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry testing of PFA-fixed human Jurkat cells with CD45RO antibody (clone T200/797); Red = isotype control, Blue = CD45RO antibody.

SDS-PAGE analysis of purified, BSA-free CD45RO antibody (clone T200/797) as confirmation of integrity and purity.