CD45RO Antibody

SKU: orb385702

Description

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: FACS, IHC-P
• Dilution Range: Flow cytometry: 0.5-1ug/10^6 cells,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Due to variation in protocol and secondary antibody used, the CD45RO antibody may need to be titered for optimal performance.1. FFPE staining requires 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: T200/797
• Immunogen: Human T lymphocytes were used as immunogen for this CD45RO antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of CD45RO antibody and FFPE human tonsil. HIER: boil tissue sections in 10mM citrate buffer, pH6 for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry testing of PFA-fixed human Jurkat cells with CD45RO antibody (clone T200/797); Red = isotype control, Blue = CD45RO antibody.

SDS-PAGE analysis of purified, BSA-free CD45RO antibody (clone T200/797) as confirmation of integrity and purity.

Quick Database Links

UniProt

P08575