CD45RB antibody

• Catalog Number: orb44073
• Category: Antibodies
• Description: Mouse Monoclonal to CD45RB.
• Clonality: Monoclonal
• Clone Number: MEM-143
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Human peripheral blood lymphocytes.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 4 μg/ml. The staining pattern of the MEM-143 shows prominent differences from other anti-CD45RB reagents including standard antibody MEM-55, mostly as a result of the recognition of differences in glycosylation. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive control: human tonsil.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD45RB
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD45RB antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 4 μg/ml. The staining pattern of the MEM-143 shows prominent differences from other anti-CD45RB reagents including standard antibody MEM-55, mostly as a result of the recognition of differences in glycosylation. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive control: human tonsil.
• Expiration Date: 12 months from date of receipt.

Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD45RB (MEM-143) purified antibody (concentration in sample 0.6 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45RB (MEM-143) purified antibody (concentration in sample 0.6 µg/ml) GAM APC.

Immunohistochemistry staining of human tonsil (paraffin sections) using anti-CD45RB (clone MEM-143).

Anti-Hu CD45RB Purified (clone MEM-143) works in WB application under reducing and non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Ramos, Molt-4, and HeLa cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody MEM-143 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed. CD45RB molecules were detected at ~180-260 kDa in Jurkat and Ramos cell line under reducing and non-reducing conditions.