CD45 / LCA (Leucocyte Marker) Antibody

• Catalog Number: orb2318585
• Category: Antibodies
• Description: CD45 / LCA (Leucocyte Marker) Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2B11 + PD7/26
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Isolated neoplastic cells from T cell lymphoma (2B11); and human peripheral blood lymphocytes maintained in T cell growth factor (PD7/26)
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Dilution range: Flow Cytometry (1-2ug/million cells); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 180-220kDa
• Target: PTPRC
• Entrez: 5788
• UniProt ID: P08575
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: B220, CD45R, GP180, Leukocyte common antigen (LCA)
• Note: For research use only
• Application notes: Positive Control: PBMCs, Jurkat, Ramos, U-698 or GA-10 cells. Tonsil and Spleen.
• Expiration Date: 12 months from date of receipt.