CD45 / LCA Antibody (aa1029-1249, clone 3G4)

• Catalog Number: orb1537930
• Category: Antibodies
• Description: CD45 / LCA Antibody (aa1029-1249, clone 3G4)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3G4
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: Recombinant human CD45 (1029-1249aa) purified from E. coli.
• Concentration: 1 mg/ml
• Dilution range: ELISA, IHC, IHC-P (10 µg/ml), WB (1:1000 - 1:3000)
• Conjugation: Unconjugated
• Target: CD45 / LCA
• Storage: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4, 0.1% Sodium Azide. PBS, pH 7.4, 0.1% Sodium Azide
• Alternative names: PTPRC, B220, CD45R, CD45, gp180, Leukocyte common
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B2941 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2941 was determined to be 10 ug/ml.
• Expiration Date: 12 months from date of receipt.