CD45 Antibody

SKU: orb639495

Description

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

Images & Validation

• Tested Applications: IHC-P
• Dilution range: Immunohistology (FFPE): 0.5-1.0ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Due to variation in protocol and secondary antibody used, the CD45 antibody may need to be titered for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: PTPRC/1460
• Immunogen: Human B lymphocytes were used as the immunogen for the CD45 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Disclaimer: For research use only

IHC test of FFPE human tonsil probed with CD45 antibody (clone PTPRC/1460). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

Quick Database Links

UniProt

P08575