CD44 Antibody

• Catalog Number: orb251516
• Category: Antibodies
• Description: CD44 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CD44 (29-56aa RFAGVFHVEKNGRYSISRTEAADLCKAF), different from the related mouse and rat sequences by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunofluorescence, 2μg/ml, Human, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 81538 MW
• UniProt ID: P16070
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: CD44 antigen;CDw44;Epican;Extracellular matrix rec
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of CD44 using anti-CD44 antibody.Lane 1:human HeLa cell;2:human U87 cell;3:rat PC-12 cell;4:rat C6 cell.

IF analysis of CD44 using anti-CD44 antibody.CD44 was detected in paraffin-embedded section of human tonsil tissues.

IF analysis of CD44 using anti-CD44 antibody.CD44 was detected in paraffin-embedded section of rat lymphaden tissues.

IHC analysis of CD44 using anti-CD44 antibody. CD44 was detected in a paraffin-embedded section of Human Intestinal Cancer tissue.