CD44 Antibody

• Catalog Number: orb1274522
• Category: Antibodies
• Description: CD44 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 148-247 of human CD44 (NP_001189486.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 82kDa
• Target: CD44
• UniProt ID: P16070
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: CD44, CD44 molecule (Indian blood group), CDW44, C
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of mouse spleen, using CD44 Antibody (orb1274522) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Western blot analysis of extracts from normal (control) and CD44 knockout (KO) HeLa cells, using CD44 antibody (orb1274522) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunofluorescence analysis of HeLa cells using CD44 antibody (orb1274522) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of A431 cells using CD44 antibody (orb1274522) at dilution of 1:200. Blue: DAPI for nuclear staining.