CD44 Antibody

• Catalog Number: orb1252799
• Category: Antibodies
• Description: CD44 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 156-3C11
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human, Monkey, Primate
• Isotype: IgG2a, kappa
• Immunogen: Stimulated human leukocytes were used as the immunogen for this CD44 / HCAM antibody.
• Concentration: 0.2 mg/mL
• Dilution range: ELISA: order BSA-free format for coatingFlow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCAM antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD44
• UniProt ID: P16070
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: CD44, CD44 molecule (Indian blood group), CDW44, C
• Note: For research use only
• Application notes: ELISA: order BSA-free format for coatingFlow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCAM antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of human tonsil stained with HCAM antibody (156-3C11).

IHC testing of human breast cancer stained with HCAM antibody (156-3C11).

SDS-PAGE Analysis of Purified, BSA-Free HCAM Antibody (clone 156-3C11). Confirmation of Integrity and Purity of the Antibody.