CD44 Antibody

• Catalog Number: orb1248325
• Category: Antibodies
• Description: CD44 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-NRDGTRYVQKGEYRT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75-82kDa band observed in lysates of cell line U-2 OS (calculated MW of 81.5kDa according to NP_000601.3 and 76.6 according to NP_001001389.1). Recommended concentration: 0.01-0.03ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 75-82kDa
• Target: CD44
• UniProt ID: P16070
• NCBI: NP_001189484.1, NP_001189486.1, NP_000601.3, NP_001001390.1, NP_001189485.1, NP_001001389.1, NP_001001391.1, NP_001001392.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CD44, CD44 molecule (Indian blood group), CDW44, C
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75-82kDa band observed in lysates of cell line U-2 OS (calculated MW of 81.5kDa according to NP_000601.3 and 76.6 according to NP_001001389.1). Recommended concentration: 0.01-0.03ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248325 (0.03 ug/ml) staining of U-2 OS lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.