CD43 Antibody [84-3C1], Rabbit IgG

• Catalog Number: orb1671628
• Category: Antibodies
• Description: CD43 Antibody [84-3C1], Rabbit IgG
• Clonality: Recombinant
• Clone Number: 84-3C1
• Tested applications: FC, IF, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunizng mice with stimulated human leukocytes.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: SPN
• UniProt ID: P16150
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Leukosialin, Sialophorin, GPL115, Galactoglycoprot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed K562 cells with anti-CD43 antibody 84-3C1. Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips stained with the chimeric r version of 84-3C1 (orb1671628) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671628- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD43 antibody 84-3C1. Daudi(A) cell lysate- K562(B) cell lysate and human bone marrow(C) tissue lysate (35 ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of 84-3C1f (orb1671628) at 0.1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow cytometry using the anti-CD43 antibody 84-3C1. K562 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r1 version of 84-3C1 (orb1671628 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.