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Catalog Number | orb1272325 |
---|---|
Category | Antibodies |
Description | CD40LG Antibody (Biotin) |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsCD40L (Human sCD40L). |
Concentration | batch dependent |
Dilution range | ELISA:Sandwich:To detect hsCD40L by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human sCD40L (XP-5263) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40L. Western Blot:To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Biotin |
Target | CD40LG |
UniProt ID | P29965 |
NCBI | P29965 |
Storage | sCD40 Ligand antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | IGM, IMD3, TRAP, gp39, CD154, CD40L, HIGM1, T-BAM, Read more... |
Note | For research use only |
Application notes | ELISA:Sandwich:To detect hsCD40L by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human sCD40L (XP-5263) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40L. Western Blot:To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
To detect hsCD40L by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human sCD40L (orb1272326) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hsCD40L.
To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
FC, ICC, IHC-Fr, IHC-P, IP, WB | |
Mouse | |
Hamster | |
Monoclonal | |
Biotin |
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