CD40L Antibody [AT161-8], Rabbit IgG

• Catalog Number: orb1671850
• Category: Antibodies
• Description: CD40L Antibody [AT161-8], Rabbit IgG
• Clonality: Recombinant
• Clone Number: AT161-8
• Tested applications: FC, IF
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising mice with Chinese hamster ovary (CHO) cells expressing human CD40L.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD154, CD40 ligand, CD-40L, CD-40 Ligand, CD40-L,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-CD40L antibody AT161-8. Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r1 version of AT161-8 (orb1671850 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of human peripheral blood monocytes using anti-MHC I antibody (AT161-8). Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of AT161-8 (orb1671850) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671850- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.