CD4 (mouse specific) Rat Monoclonal Antibody

SKU: orb1294280

Description

Rat Monoclonal antibody to CD4 (mouse specific)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IF
• Dilution Range: Immuno fluorescence 1:100-1:200, Flow Cytometry 1:50-1:100
• Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Clone No.: BTO6X7
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Mouse CD4
• Target: CD4 (mouse specific)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294280 anti-CD4 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

CD4 antibody, orb1294280, Sample: C57BL/6 mouse splenocytes.

Quick Database Links

Gene Symbol

CD4 (mouse specific)