CD4 Antibody

SKU: orb1239038

Description

CD4 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 8G1B12
• Immunogen: Mouse monoclonal CD4 antibody (orb1239038) was raised against a 193 amino acid recombinant protein near the amino terminus of human CD4.
• Target: CD4
• Molecular Weight: Predicted: 51kDObserved: 51 kD
• Purification: CD4 Monoclonal Antibody is Protein A purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: CD4 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD4 Antibody [8G1B12] : CD4mut

Western Blot Validation in Rat Lung Tissue Lysate.Loading: 15 μg of lysates per lane. Antibodies: CD4 orb1239038 (A: 0.5 μg/mL and B: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.

Western Blot Validation in Human, Mouse and Rat Tissue Lysate.Loading: 15 μg of lysates per lane. Antibodies: CD4 orb1239038 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.

Immunohistochemistry Validation of CD4 in Human Thymus Tissue.Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-CD4 antibody (orb1239038) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-mouse IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of CD4 in Human Thymus Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed human thymus tissue labeling CD4 with orb1239038 at 5 μg/mL, followed by goat anti-mouse IgG secondary antibody at 1/500 dilution (green), Phylloidin staining (red) and DAPI staining (blue).

Quick Database Links

Gene Symbol

CD4

UniProt

P01730

RefSeq (Protein):NP_000607

Roham Deyhimfar 1, Keykavos Gholami 2, Iman Menbari Oskouie 1, Leila Zareian Baghdadabad 1, Parisa Zahmatkesh 1, Mohammadreza Shoghi 3, Gholamreza Mesbah 4, Fateme Guitynavard 1, Seyed Mohammad Kazem Aghamir 5 High-dose vitamin C improves BCG immunotherapy's efficacy in a murine ectopic model of bladder cancer Pathol Res Pract ., (2025)

PubMed: 40907423

Applications

IHC

Reactivity

Mouse