CD35 antibody

• Catalog Number: orb154405
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD35
• Clonality: Monoclonal
• Clone Number: E11
• Tested applications: FC, ICC, IHC-Fr, IHC-P, IP
• Reactivity: Human, Primate
• Isotype: Mouse IgG1
• Immunogen: Acute monocytic leukemia cells and normal blood monocytes
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): Heat mediated antigen retrieval. Immunohistochemistry (frozen sections): Acetone fixation. Flow cytometry: Recommended dilution: 1-4 µg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD35
• Entrez: 1378
• UniProt ID: P17927
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti C3 binding protein antibody, anti C3b/C4b rec
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Heat mediated antigen retrieval. Immunohistochemistry (frozen sections): Acetone fixation. Flow cytometry: Recommended dilution: 1-4 µg/ml
• Expiration Date: 12 months from date of receipt.

Separation of human CD35 positive lymphocytes (red-filled) from CD35 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD35 (E11) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD35 (E11) purified antibody (concentration in sample 3 μg/ml, GAM APC).