CD34 Antibody

• Catalog Number: orb1672254
• Category: Antibodies
• Description: CD34 Antibody
• Clonality: Recombinant
• Clone Number: My10
• Tested applications: FC, IHC, IP
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: KG-1a Human myeloblastic leukemic cell line (immature human myeloid cell line) from a patient with nonlymphocytic leukemia.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CD34
• UniProt ID: P28906
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Hematopoietic progenitor Ag, Hematopoietic progeni
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (3.0 - panel A) or the rmeric version of My10 (orb1672254 - panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody and cells analysed on a FACSCanto flow-cytometer.

Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rmeric version of My10 (orb1672254). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer. Primary antibody incubation with orb1672254 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody for 20 mins followed by Ddiaminobenzidine- and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration- 2-4 ug/ml.

Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35 ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of My10 (orb1672254) at 0.1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa- tho ugh a second isoform at 35.2kDa is also present- with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. orb1672254 successfully detected both isoforms of human CD34.