CD33 monoclonal antibody (M01), clone 2D12-G4

• Catalog Number: orb2294816
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant CD33.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: CD33 (AAH28152.1, 1 a.a. ~ 364 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MPLLLLLPLLWAGALAMDPNFWLQVQESVTVQEGLCVLVPCTFFHPIPYYDKNSPVHGYWFREGAIISGDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVHGAIGGAGVTALLALCLCLIFFIVKTHRRKAARTAVGRNDTHPTTGSASPKHQKKSKLHGPTETSSCSGAAPTVEMDEELHYASLNFHGMNPSKDTSTEYSEVRTQ
• Tested applications: ELISA, WB
• Clone Number: 2D12-G4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: AAH28152.1

Western Blot detection against Immunogen (65.78 KDa).

CD33 monoclonal antibody (M01), clone 2D12-G4 Western Blot analysis of CD33 expression in HL-60.

Western Blot analysis of CD33 expression in transfected 293T cell line by CD33 monoclonal antibody (M01), clone 2D12-G4. Lane 1: CD33 transfected lysate(39.7 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of CD33 over-expressed 293 cell line, cotransfected with CD33 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CD33 monoclonal antibody (M01), clone 2D12-G4 (Cat # orb2294816). GAPDH (36.1 kDa) used as specificity and loading control.