CD325 Antibody (PE)

• Catalog Number: orb1786026
• Category: Antibodies
• Description: CD325 (N-cadherin) is a type I transmembrane protein, which forms a complex with catenins, that is linked to the actin cytoskeleton. This complex is important in synapses and for functional plasticity of neurons, and is also essential for embryonic development. Decreased CD325 cleavage caused by mutations in presenilin 1 is associated with Alzheimer´s disease. Besides nervous system, CD325 is expressed on the surface of malignant T cells, and increases their adhesion to epithelia, as well as their ability to invade and metastasize to inflammatory sites.
• Clonality: Monoclonal
• Clone Number: 8C11
• Tested applications: FC
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1 kappa
• Immunogen: bacterially expressed extracellular domain of human CD325
• Conjugation: PE
• Target: CD325
• Entrez: 1000
• UniProt ID: P19022
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: N-Cadherin, cadherin 2, CDHM, CDH2
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of HeLa cells stained using anti-human CD325 (8C11) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as CD325 APC concentration, black-dashed) in flow cytometry analysis (surface staining).