CD32 antibody

• Catalog Number: orb222729
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD32
• Clonality: Monoclonal
• Clone Number: 3D3
• Tested applications: CyTOF, FC
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: purified glycosylated recombinant human FcgammaRIIa2
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 2-6 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD32
• Entrez: 2212
• UniProt ID: P12318
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: FcgammaRII
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 2-6 μg/ml.
• Expiration Date: 12 months from date of receipt.

Mass cytometry (surface staining) of human peripheral blood with anti-CD32 (3D3) 167Er. Gated on singlets.

Separation of human CD32 positive lymphocytes (red-filled) from CD32 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD32 (3D3) purified antibody (concentration in sample 1.78 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD32 (3D3) purified antibody (concentration in sample 1.78 µg/ml, GAM APC).