CD32 Antibody

• Catalog Number: orb1821645
• Category: Antibodies
• Description: CD32 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MDE-8
• Tested applications: Blocking, ELISA, FC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: The original antibody was generated by immunizing Human Ig-Tg (Co7) mice with FcgammaRIIa-H131 conjugated to human serum albumin in complete Freund’s adjuvant.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: CD32
• UniProt ID: P12318
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CDw32; Fc-gamma RII-a; FcγRIIa; FcγRII; FcRII-a; F
• Note: For research use only
• Application notes: The binding ability of MDE-8 to FcgammaRIIa was confirmed using flow cytometry. MDE-8 binds to FcgammaRIIa on cell lines, peripheral bloodmonocytes, PMN, platelets and blocked ligand binding via this receptor. It can also effectively blocked FcγRIIa ligand-binding via its F(ab')2 fragment. Overnight incubation of monocytes with F(ab')2 fragments of MDE-8 leads to a 60% decrease in cell surface expression of FcγRIIa. MDE-8 whole antibody induced a concomitant 30% decrease of FcγRI on THP-1cells and monocytes (PMID: 15982355). MDE-8 was reoported to induce Fc-mediated hypothermia and severe thrombocytopenia in FCGR2A mice (PMID: 26396093).
• Expiration Date: 12 months from date of receipt.