CD32 Antibody

• Catalog Number: orb1821643
• Category: Antibodies
• Description: CD32 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MDE-9
• Tested applications: Blocking, ELISA, FC, IF, IHC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: The original antibody was generated by immunizing Human Ig-Tg (Co7) mice with FcgammaRIIa-H131 conjugated to human serum albumin in complete Freund’s adjuvant.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: CD32
• UniProt ID: P12318
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CDw32; Fc-gamma RII-a; FcγRIIa; FcγRII; FcRII-a; F
• Note: For research use only
• Application notes: MDE-9 was able to detect FcgammaRIIa-H131 in both flow cytometry and immunohistochemistry. MDE-9 bound FcgRIIa-H131 on cell lines expressing FcgRIIaH131, as well as on peripheral blood monocytic cells and PMN in immunofluorescence assays. MDE-9 was used to develop an FcgammaRIIa allotyping method based on flow cytometry. The results of flow cytometric FcgammaRIIa allotyping correlated completely with PCR genotyping. This novel allotyping assay can facilitate the screening of patients in a routine diagnostic setting. A combination of MDE-9 and mAb 41H16 can be used in FcgammaRIIa-H/H131 homozygous individuals to detect FcgammaRIIa and FcgammaRIIb surface expression on monocytes (PMID: 15604023).
• Expiration Date: 12 months from date of receipt.