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Catalog Number | orb639654 |
---|---|
Category | Antibodies |
Description | This CD32 antibody binds most strongly to the IIa isoforms of CD32 (FcRII), a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | FCGR2A/479 |
Tested applications | FACS |
Reactivity | Human |
Isotype | Mouse IgG2b, kappa |
Immunogen | Recombinant human protein was used as the immunogen for the CD32 antibody. |
Dilution range | Flow cytometry: 1-2ug/million cells in 0.1ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This CD32 antibody is available for research use only. |
UniProt ID | P12318 |
Storage | Store the CD32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
Functional Studies, IF | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
Functional Studies, IF | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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