CD315 Antibody (PE)

• Catalog Number: orb1806409
• Category: Antibodies
• Description: CD315, also known as prostaglandin F2 receptor negative regulator, is an approximately 135 kDa transmembrane glycoprotein, which associates with actin cytoskeleton, and with CD9 and CD81, but not with other tetraspanins. It seems to be involved in regulation of cell polarity and motility. CD315 is expressed mainly by keratinocytes, activated monocytes, and a subset of B cells, but it can be also used for distinguishing between strongly positive colon cancer and fibrosarcoma cells, and their negative normal cell counterparts.
• Clonality: Monoclonal
• Clone Number: 1F11
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: HeLa cells
• Conjugation: PE
• Target: CD315
• Entrez: 5738
• UniProt ID: Q9P2B2
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: PF2RI, SMAP-6, PTGFRN, FPRP, EWI-F
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of A431 cells stained using anti-human CD315 (1F11) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from A431 cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as CD315 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of A431 cell suspension.