CD314 antibody (FITC)

• Catalog Number: orb154477
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to FITC which recognizes CD314 that is also known as BAT221, NKG2D a cell surface receptor of the type II transmembrane receptor NKG2 family. CD314 is expressed on NK cells and some T cell subsets, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion.
• Clonality: Monoclonal
• Clone Number: 1D11
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: NKL cell line
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD314
• Entrez: 22914
• UniProt ID: P26718
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-NKG2D antibody, anti-KLRK1 antibody, anti-KLR
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD314 (1D11) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood) and anti-human CD56 (LT56) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Separation of human CD314 positive CD56 positive NK cells (red-filled) from CD314 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD314 (1D11) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD314 (1D11) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood).