CD307a Antibody (PE)

• Catalog Number: orb1467378
• Category: Antibodies
• Description: CD307a is a type I transmembrane glycoprotein of the Fc receptor family. It contains two ITAM motifs in its cytoplasmic domain. CD307a is expressed mainly on the surface of mature B-cells, and is down-regulated in germinal center B-cells. Expression of CD307a is higher in patients with autoimmune diseases, compared with healthy controls.
• Clonality: Monoclonal
• Clone Number: E3
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: DNA-immunization followed by a boost with the CD307a transfected cells
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD307a
• Hazard Information: Non-Toxic
• Entrez: 115350
• UniProt ID: Q96LA6
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: FcRL1, FcRH1, IRTA5
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD307a(E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (4G7) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD19 positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD307a (E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD307a (E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).