CD3e Antibody / Cytoplasmic domain

SKU: orb750081

Description

Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FACS, IF, WB
• Dilution Range: Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml for 60 min at RT
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Optimal dilution of the CD3e antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: PC3/188A
• Immunogen: Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P07766