CD3 epsilon Antibody

• Catalog Number: orb750081
• Category: Antibodies
• Description: Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
• UniProt ID: P07766
• Tested applications: FACS, IF, WB
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml for 60 min at RT
• Application notes: Optimal dilution of the CD3e antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PC3/188A
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This CD3e antibody is available for research use only.
• Note: For research use only

IHC testing of human tonsil tissue with CD3e antibody (clone PC3/188A). Required HIER: boil tissue sections in 10mM citrate buffer, pH6.0, for 10-20 min.

Western blot testing of human Jurkat cell lysate with CD3e antibody (clone PC3/188A). Expected molecular weight ~23 kDa.

SDS-PAGE Analysis of Purified, BSA-Free CD3e Antibody (clone PC3/188A). Confirmation of Integrity and Purity of the Antibody.

Western blot testing of human thymus lysate with CD3e antibody (clone PC3/188A). Expected molecular weight ~23 kDa.