CD3 delta Antibody / CD3D

• Catalog Number: orb2636373
• Category: Antibodies
• Description: The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of g and e chains (ge), a heterodimer of d and e chains (de) and a homodimer of two z chains (zz) or a heterodimer of z and h chains (zh). The z and h chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The g, e and d chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, the z chain contains three consecutive copies of the same motif. Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity. The crystal structure of the ZAP-70 SH2 domains bound to the z chain ITAMs has been solved.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CD3D/8898
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant fragment (within amino acids 1-171) of human CD3D protein was used as the immunogen for the CD3 delta antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CD3 delta antibody is available for research use only.
• UniProt ID: P04234
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the CD3 delta antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil tissue with CD3 delta antibody (clone CD3D/8898). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free CD3 delta antibody (clone CD3D/8898) as confirmation of integrity and purity.