CD3 antibody (PerCP)

• Catalog Number: orb44565
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD3 conjugated to PerCP
• Clonality: Monoclonal
• Clone Number: UCHT1
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG1
• Immunogen: human thymocytes followed by Sezary T cells
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
• Purity: Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PerCP
• Target: CD3
• Entrez: 916
• UniProt ID: P07766
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti CD 3 antibody (PerCP), anti Cd247 antibody (P
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
• Expiration Date: 12 months from date of receipt.

References

Rudolf-Oliveira, Renata Cristina Messores et al. Determination of normal expression patterns of CD86, CD210a, CD261, CD262, CD264, CD358, and CD361 in peripheral blood and bone marrow cells by flow cytometry Immunol Lett, 194, 44-55 (2018)

PubMed: 29274771

Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (UCHT1) PerCP antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (UCHT1) PerCP antibody (10 µl reagent / 100 µl of peripheral whole blood).