CD3 antibody (PE)

• Catalog Number: orb44680
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD3 conjugated to PE
• Clonality: Monoclonal
• Clone Number: APA1/1
• Tested applications: FC
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1
• Immunogen: Purified human CD3 proteins isolated from thymus
• Concentration: 0.1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.Immunocytochemistry: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD3 epsilon (activation epitope)
• Entrez: 916
• UniProt ID: P07766
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti CD 3 antibody (PE), anti Cd247 antibody (PE),
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.Immunocytochemistry: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells.
• Expiration Date: 12 months from date of receipt.

Separation of Jurkat cells stained using anti-CD3 epsilon activation epitope (APA1/1) PE antibody (concentration in sample 3 µg/ml, red) from unstained Jurkat cells (grey) in flow cytometry analysis (intracellular staining).