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Catalog Number | orb507486 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against CD3 activation epitope conjugated to APC |
Clonality | Monoclonal |
Clone Number | APA1/1 |
Tested applications | FC |
Reactivity | Human, Mouse |
Isotype | Mouse IgG1 |
Immunogen | Purified human CD3 proteins isolated from thymus |
Concentration | 0.1 mg/ml |
Dilution range | Flow cytometry: Recommended dilution: 1-2 μg/ml. Intracellular staining. |
Purity | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | APC |
Target | CD3 epsilon (activation epitope) |
Entrez | 916 |
UniProt ID | P07766 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-CD3 epsilon chain antibody, anti-T3E antibody Read more... |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 1-2 μg/ml. Intracellular staining. |
Expiration Date | 12 months from date of receipt. |
Separation of Jurkat cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis (intracellular staining) stained using anti-human CD3 activation epitope (APA1/1) APC antibody (concentration in sample 2 μg/ml).
Flow cytometry intracellular staining pattern of Jurkat cell suspension using anti-human CD3 activation epitope (APA1/1) APC antibody (concentration in sample 2 μg/ml).
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