CD28 antibody (PE )

• Catalog Number: orb154508
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to PE which recognizes CD28 that is expressed constitutively on virtually 100% of mouse Tcells and on developing thymocytes. . Activation and proliferation of T cells can be induced by interaction of the T cell receptor with peptide-MHC complexes expressed on the surface of APCs.
• Clonality: Monoclonal
• Clone Number: JJ319
• Tested applications: FC
• Reactivity: Rat
• Isotype: Mouse IgG1 kappa
• Immunogen: Mouse A20J B lymphoma cells transfected with rat CD28
• Concentration: 0.5 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD28
• Entrez: 25660
• UniProt ID: P31042
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Tp44 antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of rat CD28 positive splenocytes (red-filled) from CD28 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-rat CD28 (JJ319) PE antibody (concentration in sample 0.56 μg/ml).

Flow cytometry surface staining pattern of rat splenocytes stained using anti-rat CD28 (JJ319) PE antibody (concentration in sample 0.56 μg/ml).