CD274 antibody (PE)

• Catalog Number: orb414546
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD274 conjugated to PE
• Clonality: Monoclonal
• Clone Number: 29E.2A3
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG2b kappa
• Immunogen: Full length human CD274
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD274
• Entrez: 29126
• UniProt ID: Q9NZQ7
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-B7H1 antibody, anti-PDL1 antibody, anti-PDCD1
• Note: For research use only
• Application notes: The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
• Expiration Date: 12 months from date of receipt.

Separation of human CD274 positive cells (red-filled) from cellular debris (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cell suspension stained using anti-human CD274 (29E.2A3) PE antibody (10 µl reagent per milion cells in 100 µl of cell suspension).

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cell suspension stained using anti-human CD274 (29E.2A3) PE antibody (10 µl reagent per milion cells in 100 µl of cell suspension).